WebApr 7, 2024 · For TRUPATH, plates were read with a BioTek Synergy Neo2 plate reader set at 37°C with a standard 400 nm emission filter and 510 nm long pass filter. For all other BRET assays, a standard 480 nm RLuc emission filter and 530 nm (for experiment using mVenus) or custom 542 nm (for experiments using mKO) long pass filter was utilized (Chroma … WebMay 4, 2024 · The first fully optimized biosensor was Gαq. We defined the initial set of Gαq-RLuc8 insertions sites to be 98–106 and 116–126 within the loops connecting αA-αB and …
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