WebAug 20, 2024 · The Flp-In system integrates a vector into cell lines at a specific genomic locus, but has not been used for integration of more than one gene. ... X. et al. … WebJan 15, 2024 · We also established CHO cells expressing the WT protein using the Flp-In system as previously described (thereafter called Flp-In CHO-WT cells) [6]. The pcDNA5/FRT-PiT-2-flag in GFP vectors were obtained by inserting GFP into Loop7 of the SLC20A2 (WT, T115 M, and R467X) using in-fusion cloning system (Takara Bio Inc.; …
Flp-In™ System Thermo Fisher Scientific - JP
WebThe Flp-In™ System allows for stable integration and expression of your gene of interest to deliver single-copy isogenic cell lines. Flp-In™ expression involves introduction of a Flp … WebNov 8, 2013 · The Flp-In CHO cell line contains a single integrated FRT site and stably expresses the lacZ-Zeocin fusion gene. Flp-In CHO cells were cultured in Ham’s F12 medium (Nissui, Tokyo, Japan) containing 100 U/ml of penicillin (Meiji Seika, Tokyo, Japan), 100 μg/ml of streptomycin (Meiji Seika), 100 μg/ml of Zeocin (Invitrogen) and 10% fetal ... how do i connect my bose earbuds
A single-plasmid vector for transgene amplification using ... - PubMed
WebFlp-In™ T-REx™-293 3 × 106 cells, frozen R780-07 Flp-In™ Host Cell Lines For your convenience, Invitrogen has available several mammalian Flp-In™ host cell lines that stably express the lacZ-Zeocin™ fusion gene from pFRT/lacZeo or pFRT/lacZeo2 (Flp-In™-CHO). Each cell line contains a single integrated FRT site WebLike Cre, FLP is a tyrosine recombinase, but it originates from the yeast Saccharomyces cerevisiae, rather than from bacteriophage. In FLP-FRT, FLP is analogous to cre and FRT to loxP – FLP recognizes the FRT sites, and catalyzes recombination between them. WebOct 25, 2024 · Flp-In-CHO:huOX40 cells as well as Flp-In-CHO:vector cells generated in-house (Pieris) were cultivated in Ham’s F12 medium (Gibco, #31765-027) with 10% FBS and 500 µg/ml hygromycin B. All mammalian cell lines were grown at 37°C and 5% CO 2. Goat anti-rabbit IgG-PE conjugate was purchased from Dianova/Jackson … how do i connect my beats headphones